{"id":4322,"date":"2025-11-05T13:53:13","date_gmt":"2025-11-05T12:53:13","guid":{"rendered":"https:\/\/sylphium.com\/webshop\/?page_id=4322"},"modified":"2025-11-05T13:56:01","modified_gmt":"2025-11-05T12:56:01","slug":"edna-sampling-strategy-field-kits","status":"publish","type":"page","link":"https:\/\/sylphium.com\/webshop\/edna-sampling-strategy-field-kits\/","title":{"rendered":"eDNA Sampling Strategy &#038; Field Kits"},"content":{"rendered":"\n<h4 class=\"wp-block-heading\">A well-designed eDNA sampling strategy is crucial to successful eDNA analysis. With the right approach, success rates of up to 95% are achievable. Sylphium Molecular Ecology provides sampling materials developed in line with the <a href=\"https:\/\/www.nen.nl\/npr-7394-2022-nl-295265\">NPR 7394<\/a> guidelines, enabling rapid and highly reliable sample collection.<\/h4>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><\/div>\n\n\n\n<figure class=\"wp-block-image alignright size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"823\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2020\/05\/Aeshna-viridis-klein.png\" alt=\"Aeshna viridis\" class=\"wp-image-2278\" style=\"width:244px;height:200px\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/05\/Aeshna-viridis-klein.png 1000w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/05\/Aeshna-viridis-klein-300x247.png 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/05\/Aeshna-viridis-klein-768x632.png 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/05\/Aeshna-viridis-klein-600x494.png 600w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/figure>\n\n\n\n<p>Choosing where to sample is essential to maximize the chance of capturing DNA from the target organism. Always consider the species\u2019 ecology and preferred microhabitats. Detection probability increases when multiple samples are collected at different locations within the same water body. Keep in mind that DNA degrades quickly\u2014at ~20&nbsp;\u00b0C, detectable eDNA can decline within ~48 hours. The full procedure and practical tips are provided in the protocol that accompanies the environmental sampling set.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">eDNA sampling strategy<\/h3>\n\n\n\n<p>Sylphium\u2019s eDNA analysis is optimized for very high analytical sensitivity (down to a single DNA molecule per analysis). In practice, the sampling strategy has the greatest impact on overall success.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Minimum volume:<\/strong> Use at least <strong>0.75\u00a0L<\/strong> per sample. Because eDNA concentrations in water are low, sufficient volume is critical. If the eDNA Dual Filter Capsule clogs before reaching this volume, use a second capsule; the filters can be combined and analyzed as a single sample in the lab.<\/li>\n\n\n\n<li><strong>Sampling spacing:<\/strong> Collect a new sample approximately every <strong>300\u00a0m<\/strong> along the water body to account for limited eDNA dispersion.<\/li>\n\n\n\n<li><strong>Sub-sampling:<\/strong> Build each sample from multiple sub-samples. Ideally, take sub-samples every <strong>~10\u00a0m<\/strong> along the shoreline to bridge the distance between main sampling points. Sub-samples of <strong>60\u00a0mL<\/strong> can be taken with the sampling kit and filtered immediately.<\/li>\n\n\n\n<li><strong>Target the preferred niche:<\/strong> Sample in microhabitats favored by the species (e.g., vegetation edges, in-\/outflows) to maximize detection probability.<\/li>\n\n\n\n<li><strong>Seasonality:<\/strong> Time your sampling to the species\u2019 life cycle and presence at the site\u2014many species are not detectable year-round.<\/li>\n<\/ul>\n\n\n\n<p>See the sampling kit <a href=\"https:\/\/sylphium.com\/webshop\/product\/syl009\/\">manual<\/a> for more recommendations and detailed instructions.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">eDNA sampling kit<\/h3>\n\n\n\n<p>The Sylphium Molecular Ecology sampling kit enables active collection and on-site filtration of water samples, minimizing contamination risk while keeping fieldwork lightweight. The kit includes an eDNA Dual Filter and a <strong>60&nbsp;mL<\/strong> syringe. Attach the filter unit to the syringe and press water through the unit\u2014no vacuum pump required. After adding preservation fluid and sealing the unit with the supplied caps, the sample remains stable for <em>weeks to months<\/em> without refrigeration. Each filter unit carries a unique sample number for secure identification and can be shipped unrefrigerated to our lab. Kits can be ordered via the <a href=\"https:\/\/sylphium.com\/webshop\/product\/syl002-environmental-sampling-kit\/\">webshop<\/a>; the kit price is deducted from the analysis fee.<\/p>\n\n\n\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"eDNA Dual Filter Capsule\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/EaQfMxnw2lQ?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\"><a href=\"https:\/\/sylphium.com\/eng\/product\/syl003-sampling-rot\/\">Sampling rod<\/a><\/h4>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1000\" height=\"750\" data-id=\"1943\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2020\/03\/YUN00002938-2.jpg\" alt=\"Sampling with rod in overgrown bank\" class=\"wp-image-1943\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/YUN00002938-2.jpg 1000w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/YUN00002938-2-300x225.jpg 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/YUN00002938-2-768x576.jpg 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/YUN00002938-2-380x285.jpg 380w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/YUN00002938-2-285x214.jpg 285w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/YUN00002938-2-600x450.jpg 600w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><figcaption class=\"wp-element-caption\">DCIM\\100MEDIA\\YUN00616.<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"888\" data-id=\"1942\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2020\/03\/IMG_20190621_101540-1-1024x888.jpg\" alt=\"Sampling rod holding sterile syringe\" class=\"wp-image-1942\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190621_101540-1-1024x888.jpg 1024w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190621_101540-1-300x260.jpg 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190621_101540-1-768x666.jpg 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190621_101540-1-1536x1333.jpg 1536w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190621_101540-1-600x521.jpg 600w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190621_101540-1.jpg 1918w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"484\" data-id=\"1941\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2020\/03\/IMG_20190910_140501-1024x484.jpg\" alt=\"Extended sampling rod at water edge\" class=\"wp-image-1941\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190910_140501-1024x484.jpg 1024w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190910_140501-300x142.jpg 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190910_140501-768x363.jpg 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190910_140501-1536x725.jpg 1536w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190910_140501-2048x967.jpg 2048w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/IMG_20190910_140501-600x283.jpg 600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/figure>\n\n\n\n<p>The custom sampling rod helps collect water in hard-to-reach areas (e.g., dense vegetation or steep banks). It securely holds a sterile 60&nbsp;mL disposable syringe (from the sampling kit), reducing the need for waders, protective suits, boats, or other equipment, and significantly speeding up fieldwork. After use, simply replace the syringe and continue sampling. The rod can be purchased separately via the <a href=\"https:\/\/sylphium.com\/eng\/product\/syl003-sampling-rot\/\">shop<\/a>.<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">Sending samples<\/h4>\n\n\n\n<p>After sampling, place the eDNA Dual Filter back into the sampling set bag with its unique sample number and ship it unrefrigerated (registered) via PostNL to:<\/p>\n\n\n\n<p><strong>Sylphium Molecular Ecology<\/strong><br>PO Box 11107<br>9700&nbsp;CC Groningen<br>The Netherlands<\/p>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"1024\" data-id=\"1787\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2020\/03\/kwabaal-1024x1024.png\" alt=\"Burbot illustration\" class=\"wp-image-1787\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-1024x1024.png 1024w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-300x300.png 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-150x150.png 150w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-768x768.png 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-120x120.png 120w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-105x105.png 105w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-570x570.png 570w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-380x380.png 380w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-285x285.png 285w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-600x600.png 600w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal-100x100.png 100w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/03\/kwabaal.png 1400w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"680\" height=\"680\" data-id=\"1148\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2020\/01\/kamsalamander.png\" alt=\"Northern crested newt\" class=\"wp-image-1148\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander.png 680w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-300x300.png 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-150x150.png 150w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-120x120.png 120w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-105x105.png 105w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-570x570.png 570w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-380x380.png 380w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-285x285.png 285w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-600x600.png 600w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/01\/kamsalamander-100x100.png 100w\" sizes=\"auto, (max-width: 680px) 100vw, 680px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"800\" height=\"800\" data-id=\"223\" src=\"https:\/\/sylphium.com\/eng\/wp-content\/uploads\/sites\/7\/2018\/12\/cyanobacteria-1.png\" alt=\"Cyanobacteria diagram\" class=\"wp-image-223\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1.png 800w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-150x150.png 150w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-300x300.png 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-768x768.png 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-120x120.png 120w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-105x105.png 105w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-570x570.png 570w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-380x380.png 380w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-285x285.png 285w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-600x600.png 600w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/12\/cyanobacteria-1-100x100.png 100w\" sizes=\"auto, (max-width: 800px) 100vw, 800px\" \/><\/figure>\n<\/figure>\n","protected":false},"excerpt":{"rendered":"<p>A well-designed eDNA sampling strategy is crucial to successful eDNA analysis. With the right approach, success rates of up to [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-4322","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>eDNA Sampling Strategy &amp; Field Kits -<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sylphium.com\/webshop\/edna-sampling-strategy-field-kits\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"eDNA Sampling Strategy &amp; Field Kits -\" \/>\n<meta property=\"og:description\" content=\"A well-designed eDNA sampling strategy is crucial to successful eDNA analysis. 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