{"id":2441,"date":"2020-12-02T10:41:35","date_gmt":"2020-12-02T09:41:35","guid":{"rendered":"https:\/\/sylphium.com\/webshop\/?post_type=product&#038;p=2441"},"modified":"2025-08-06T13:08:47","modified_gmt":"2025-08-06T11:08:47","slug":"syl111","status":"publish","type":"product","link":"https:\/\/sylphium.com\/webshop\/product\/syl111\/","title":{"rendered":"Rhodeus amarus qPCR detection kit"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-2444\" src=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling-300x300.png\" alt=\"\" width=\"300\" height=\"300\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling-300x300.png 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling-150x150.png 150w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling-768x768.png 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling-600x600.png 600w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling-100x100.png 100w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling.png 900w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><strong>The <em>Rhodeus amarus<\/em> detection kit comes with then new <\/strong><a href=\"https:\/\/sylphium.com\/webshop\/product\/etaq-qpcr-master-mix\/\"><strong>eDNA qPCR hot start mix<\/strong><\/a><strong>. This qPCR hot start mix provides excellent performance for sensitive, robust and accurate qPCR detection assay.\u00a0 The eDNA qPCR hot start mix is \u200b\u200bhighly resistant to inhibitory factors, such as humic acids present in environmental samples. <\/strong><strong>This kit contains primers and a probe to detect a highly specific region on the mitochondrial DNA of the species <em>Rhodeus amarus<\/em>.<\/strong><\/p>\n<p><strong>Detection as a service:<\/strong><\/p>\n<p>We offer the detection of <em>Rhodeus amarus <\/em>also as a service. See our eDNA service website (in <a href=\"https:\/\/sylphium.com\/eng\">English<\/a> or in <a href=\"https:\/\/sylphium.com\/edna\">Dutch<\/a>) or <a href=\"https:\/\/sylphium.com\/webshop\/contact\/\">contact us\u00a0<\/a>for more information about sampling, sending and pricing.<\/p>\n<p><strong>Common species names:<\/strong><\/p>\n<ul>\n<li>English: European bitterling<\/li>\n<li>German: Bitterling<\/li>\n<li>French: <em>Rhodeus amarus<\/em><\/li>\n<li>Dutch: Bittervoorn<\/li>\n<\/ul>\n<p><strong>Kit properties:<\/strong><\/p>\n<p>The <em>Rhodeus amarus<\/em> detection kit has:<\/p>\n<ul>\n<li><strong>High resistance to inhibiting factors<\/strong>. Environmental DNA (eDNA) extracts contain often multiple qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.<\/li>\n<li><strong>Strong fluorescence signal with low background noise<\/strong>. Isolated environmental samples contain residues of naturally occurring auto fluoresce substances that will interfere with the measurements. Environmental samples needs a strong fluorescence signal from the assay in order not to experience background interference.<\/li>\n<li><strong>Highest possible sensitivity<\/strong> (1 DNA copy per reaction). Environmental water samples may contain very low amounts of target DNA.<\/li>\n<li><strong>100% specificity<\/strong>. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc. Not only animals from the same order (fish, amphibians, reptiles, mammals, etc.) must be taken in account during primer\/probe design, but all known DNA sequences must be checked for nonspecific binding of the primers and probe.<\/li>\n<\/ul>\n<ul>\n<li><strong>\u2265 95% detection probability. <\/strong>The maximum probability of detection can only be achieved with <a href=\"https:\/\/sylphium.com\/webshop\/product\/syl009\/\">the correct sampling method<\/a>.<\/li>\n<\/ul>\n<p>The full method validation report can be found below under <strong>\u201cRelevant<\/strong> <strong>documents<\/strong>\u201d<\/p>\n<p>The kit has been developed and optimized for use on eDNA isolates purified using the Sylphium Molecular Ecology eDNA Isolation Kit (#SYL002), although other eDNA isolation methods\/kits can be used as well. If you need more information on using the obtained isolates from other methods\/kits to get reliable results, send an e-mail to <a href=\"mailto:info@sylphium.com\">info@sylphium.com<\/a>.<\/p>\n<p><strong>Kit contents:<\/strong><\/p>\n<ul>\n<li>Positive control (cloned <em>Rhodeus amarus<\/em> DNA)<\/li>\n<li>Primer\/probe mix (10x) for detection of <em>Rhodeus amarus<\/em> (FAM dye)<\/li>\n<li>eDNA qPCR master mix:\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>eTaq DNA polymerase<\/li>\n<li>eTaq qPCR mix<\/li>\n<li>PCR water<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>The kit contains all materials for 200 reactions of 25\u00b5l.<\/p>\n<h3>Relevant Documents<\/h3>\n<ul>\n<li>&#x1f4d6; <a href=\"https:\/\/sylphium.com\/downloads\/SYL111-manual.pdf\" target=\"_blank\" rel=\"noopener\">Manual<\/a><\/li>\n<li>&#x1f4d6; <a href=\"https:\/\/sylphium.com\/downloads\/SYL1003-manual.pdf\" target=\"_blank\" rel=\"noopener\">Universal protocol for eDNA qPCR master mix<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>200 reactions<\/li>\n<li><a href=\"https:\/\/sylphium.com\/webshop\/product\/etaq-qpcr-master-mix\/\">Comes with the new eDNA qPCR hot start mix for environmental samples<\/a><\/li>\n<\/ul>\n","protected":false},"featured_media":2444,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}}},"product_brand":[],"product_cat":[22,23,10],"product_tag":[],"class_list":{"0":"post-2441","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-fish","7":"product_cat-protected-and-rare-species","8":"product_cat-qpcr","9":"desktop-align-left","10":"tablet-align-left","11":"mobile-align-left","13":"first","14":"instock","15":"taxable","16":"shipping-taxable","17":"purchasable","18":"product-type-simple"},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Rhodeus amarus qPCR detection kit -<\/title>\n<meta name=\"description\" content=\"qPCR detection kit specific for Rhodeus amarus. The kit gives within 2hrs. reliable results.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sylphium.com\/webshop\/product\/syl111\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rhodeus amarus qPCR detection kit -\" \/>\n<meta property=\"og:description\" content=\"qPCR detection kit specific for Rhodeus amarus. The kit gives within 2hrs. reliable results.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/sylphium.com\/webshop\/product\/syl111\/\" \/>\n<meta property=\"article:modified_time\" content=\"2025-08-06T11:08:47+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling.png\" \/>\n\t<meta property=\"og:image:width\" content=\"900\" \/>\n\t<meta property=\"og:image:height\" content=\"900\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/\",\"url\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/\",\"name\":\"Rhodeus amarus qPCR detection kit -\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/wp-content\\\/uploads\\\/sites\\\/6\\\/2020\\\/12\\\/Bitterling.png\",\"datePublished\":\"2020-12-02T09:41:35+00:00\",\"dateModified\":\"2025-08-06T11:08:47+00:00\",\"description\":\"qPCR detection kit specific for Rhodeus amarus. The kit gives within 2hrs. reliable results.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/#primaryimage\",\"url\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/wp-content\\\/uploads\\\/sites\\\/6\\\/2020\\\/12\\\/Bitterling.png\",\"contentUrl\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/wp-content\\\/uploads\\\/sites\\\/6\\\/2020\\\/12\\\/Bitterling.png\",\"width\":900,\"height\":900},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/product\\\/syl111\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Shop\",\"item\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/shop\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Rhodeus amarus qPCR detection kit\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/#website\",\"url\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/\",\"name\":\"Sylphium molecular ecology\",\"description\":\"The eDNA company\",\"publisher\":{\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/#organization\",\"name\":\"Sylphium molecular ecology\",\"url\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/i0.wp.com\\\/sylphium.com\\\/webshop\\\/wp-content\\\/uploads\\\/sites\\\/6\\\/2018\\\/11\\\/Logo-Sylphium-molecular-ecology-transparant-klein.png?fit=1000%2C1000&ssl=1\",\"contentUrl\":\"https:\\\/\\\/i0.wp.com\\\/sylphium.com\\\/webshop\\\/wp-content\\\/uploads\\\/sites\\\/6\\\/2018\\\/11\\\/Logo-Sylphium-molecular-ecology-transparant-klein.png?fit=1000%2C1000&ssl=1\",\"width\":1000,\"height\":1000,\"caption\":\"Sylphium molecular ecology\"},\"image\":{\"@id\":\"https:\\\/\\\/sylphium.com\\\/webshop\\\/#\\\/schema\\\/logo\\\/image\\\/\"}}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Rhodeus amarus qPCR detection kit -","description":"qPCR detection kit specific for Rhodeus amarus. The kit gives within 2hrs. reliable results.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/sylphium.com\/webshop\/product\/syl111\/","og_locale":"en_US","og_type":"article","og_title":"Rhodeus amarus qPCR detection kit -","og_description":"qPCR detection kit specific for Rhodeus amarus. The kit gives within 2hrs. reliable results.","og_url":"https:\/\/sylphium.com\/webshop\/product\/syl111\/","article_modified_time":"2025-08-06T11:08:47+00:00","og_image":[{"width":900,"height":900,"url":"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling.png","type":"image\/png"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/sylphium.com\/webshop\/product\/syl111\/","url":"https:\/\/sylphium.com\/webshop\/product\/syl111\/","name":"Rhodeus amarus qPCR detection kit -","isPartOf":{"@id":"https:\/\/sylphium.com\/webshop\/#website"},"primaryImageOfPage":{"@id":"https:\/\/sylphium.com\/webshop\/product\/syl111\/#primaryimage"},"image":{"@id":"https:\/\/sylphium.com\/webshop\/product\/syl111\/#primaryimage"},"thumbnailUrl":"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling.png","datePublished":"2020-12-02T09:41:35+00:00","dateModified":"2025-08-06T11:08:47+00:00","description":"qPCR detection kit specific for Rhodeus amarus. The kit gives within 2hrs. reliable results.","breadcrumb":{"@id":"https:\/\/sylphium.com\/webshop\/product\/syl111\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/sylphium.com\/webshop\/product\/syl111\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/sylphium.com\/webshop\/product\/syl111\/#primaryimage","url":"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling.png","contentUrl":"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2020\/12\/Bitterling.png","width":900,"height":900},{"@type":"BreadcrumbList","@id":"https:\/\/sylphium.com\/webshop\/product\/syl111\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/sylphium.com\/webshop\/"},{"@type":"ListItem","position":2,"name":"Shop","item":"https:\/\/sylphium.com\/webshop\/shop\/"},{"@type":"ListItem","position":3,"name":"Rhodeus amarus qPCR detection kit"}]},{"@type":"WebSite","@id":"https:\/\/sylphium.com\/webshop\/#website","url":"https:\/\/sylphium.com\/webshop\/","name":"Sylphium molecular ecology","description":"The eDNA company","publisher":{"@id":"https:\/\/sylphium.com\/webshop\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/sylphium.com\/webshop\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/sylphium.com\/webshop\/#organization","name":"Sylphium molecular ecology","url":"https:\/\/sylphium.com\/webshop\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/sylphium.com\/webshop\/#\/schema\/logo\/image\/","url":"https:\/\/i0.wp.com\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/11\/Logo-Sylphium-molecular-ecology-transparant-klein.png?fit=1000%2C1000&ssl=1","contentUrl":"https:\/\/i0.wp.com\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2018\/11\/Logo-Sylphium-molecular-ecology-transparant-klein.png?fit=1000%2C1000&ssl=1","width":1000,"height":1000,"caption":"Sylphium molecular ecology"},"image":{"@id":"https:\/\/sylphium.com\/webshop\/#\/schema\/logo\/image\/"}}]}},"_links":{"self":[{"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/product\/2441","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/media\/2444"}],"wp:attachment":[{"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/media?parent=2441"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/product_brand?post=2441"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/product_cat?post=2441"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sylphium.com\/webshop\/wp-json\/wp\/v2\/product_tag?post=2441"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}