{"id":2576,"date":"2022-01-19T09:21:51","date_gmt":"2022-01-19T08:21:51","guid":{"rendered":"https:\/\/sylphium.com\/webshop\/?post_type=product&#038;p=2576"},"modified":"2026-01-09T16:54:38","modified_gmt":"2026-01-09T15:54:38","slug":"syl1003","status":"publish","type":"product","link":"https:\/\/sylphium.com\/webshop\/product\/syl1003\/","title":{"rendered":"eDNA qPCR hot start mix"},"content":{"rendered":"<p>The environmental\u00a0 DNA qPCR hot start mix offers outstanding performance for sensitive, robust and precise probe-based qPCR detection and quantitation of target eDNA extracted from environmental samples. eDNA qPCR hot start mix is highly resistant to inhibiting factors, like humic acids in environmental samples.<\/p>\n<hr \/>\n<h3>The eDNA qPCR hot start mix has:<\/h3>\n<ul>\n<li><strong>Highest resistance to inhibiting factors in comparison to other qPCR master mixes. <\/strong>Environmental DNA extracts contain often multiple qPCR inhibiting factors. Normal qPCR master mixes are sensitive to these substances.<\/li>\n<li><strong>Highest fluorescence signal with low background noise<\/strong>. Isolated environmental samples contain residues of naturally occurring auto fluoresce substances that will interfere with the measurements. A strong fluorescence signal from the analyses is required for this kind of samples.<\/li>\n<li><strong>Highest possible sensitivity (1 DNA copy per reaction)<\/strong>. Environmental water samples may contain very low amounts of target DNA.<\/li>\n<li><strong>Hot-start properties. <\/strong>The DNA polymerase enzyme has been genetically modified to have similar or better hot-start properties when compared to antibody-mediated hot-start enzymes. Working on ice is not necessary during the preparation of the PCR mixture.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_4637\" aria-describedby=\"caption-attachment-4637\" style=\"width: 678px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4637 \" src=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/eTaq-vs-EMM.png\" alt=\"\" width=\"678\" height=\"283\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/eTaq-vs-EMM.png 912w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/eTaq-vs-EMM-300x125.png 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/eTaq-vs-EMM-768x321.png 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/eTaq-vs-EMM-600x251.png 600w\" sizes=\"auto, (max-width: 678px) 100vw, 678px\" \/><figcaption id=\"caption-attachment-4637\" class=\"wp-caption-text\">CT values of eTaq qPCR master mix and EMM at different humic acid concentrations with a fixed amount of target copies. Lower CT values are better. EMM gave no fluorescent signal at 1000 ng\/\u00b5l humic acids.<\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<figure id=\"attachment_2581\" aria-describedby=\"caption-attachment-2581\" style=\"width: 573px\" class=\"wp-caption alignnone\"><a href=\"https:\/\/sylphium.com\/webshop\/?attachment_id=2581\" rel=\"attachment wp-att-2581\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-2581\" src=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration-1024x541.jpg\" alt=\"Logarithmic fluorescent output curves with eTaq qPCR master mix based on 10^6, 10^5, 10^4, 10^3, 100, 10 and 1 target DNA copies per 25 \u00b5l reaction mix in triplicate.\" width=\"573\" height=\"303\" srcset=\"https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration-1024x541.jpg 1024w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration-300x158.jpg 300w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration-768x406.jpg 768w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration-1536x811.jpg 1536w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration-600x317.jpg 600w, https:\/\/sylphium.com\/webshop\/wp-content\/uploads\/sites\/6\/2022\/01\/calibration.jpg 1787w\" sizes=\"auto, (max-width: 573px) 100vw, 573px\" \/><\/a><figcaption id=\"caption-attachment-2581\" class=\"wp-caption-text\">Logarithmic fluorescent output curves with eDNA qPCR hot start mix based on 10^6, 10^5, 10^4, 10^3, 100, 10 and 1 target DNA copies per 25 \u00b5l reaction mix in triplicate.<\/figcaption><\/figure>\n<h3><\/h3>\n<h3>Kit content<\/h3>\n<ul>\n<li>2x 2500 \u00b5l eTaq qPCR mix (2x)<\/li>\n<li>2x 200 \u00b5l eTaq DNA polymerase<\/li>\n<\/ul>\n<h3><\/h3>\n<h3>Relevant Documents<\/h3>\n<ul>\n<li><a href=\"https:\/\/sylphium.com\/downloads\/SYL1003-protocol.pdf\">Protocol eDNA qPCR hot start mix (SYL1003)<\/a><\/li>\n<li><a href=\"https:\/\/sylphium.com\/downloads\/SYL1003-validation.pdf\">Validation report eDNA qPCR hot start mix (SYL1003)<\/a><\/li>\n<li>Material safety data sheets: <a href=\"https:\/\/sylphium.com\/downloads\/SYL1003-MSDS-1.pdf\">Sylphium eTaq qPCR mix<\/a>, <a href=\"https:\/\/sylphium.com\/downloads\/SYL1003-MSDS-1.pdf\">Sylphium eTaq DNA polymerase<\/a><\/li>\n<\/ul>\n<h3><\/h3>\n<h3>References<\/h3>\n<p>* Uchii, K., Doi, H., Okahashi, T., Katano, I., Yamanaka, H., Sakata, M. K., et al. (2019). Comparison of inhibition resistance among PCR reagents for detection and quantification of environmental DNA. <em> DNA<\/em> 1, 359\u2013367.<\/p>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>400 reactions<\/li>\n<li>eDNA qPCR hot start mix is included in all qPCR detection kits of Sylphium<\/li>\n<\/ul>\n","protected":false},"featured_media":4636,"template":"","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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