All Sylphium sample sets include an Internal Positive Control (IPC) within the preservation solution, located in the small syringe of the sampling set (SYL009). The IPC serves as an efficiency check for the DNA isolation procedure, ensures the quality and purity of the isolated eDNA, and helps prevent false negative results. This internal control consists of a small piece of synthetic xenobiotic DNA. The selected positive control DNA sequence is not found in the aquatic environment and does not interfere with the detection of target organisms. Additionally, the IPC Quantification Kit can be ordered separately or together with the eDNA Isolation Kit. The IPC is detected through the FAM channel.<\/p>\n
Kit properties:<\/strong><\/p>\n The\u00a0IPC qPCR quantification kit has:<\/p>\n The full method validation report can be found below under \u201cRelevant<\/strong> documents<\/strong>\u201d<\/p>\n The kit has been developed and optimized for use on eDNA isolates purified using the Sylphium Molecular Ecology eDNA Isolation Kit (#SYL002), although other eDNA isolation methods\/kits can be used as well. If you need more information on using the obtained isolates from other methods\/kits to get reliable results, send an e-mail to info@sylphium.com<\/a>.<\/p>\n Kit contents:<\/strong><\/p>\n The kit contains all materials for 200 reactions of 25\u00b5l.<\/p>\n\n
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Relevant Documents<\/h3>\n