Information

---

The SYL149 Lissotriton vulgaris qPCR Detection kit has been developed for accurate and consistent environmental DNA (eDNA) detection. Together with the Sylphium eDNA qPCR Mix, this kit delivers exceptional sensitivity, robustness, and accuracy in qPCR-based detection assays. The eDNA qPCR Mix is highly resistant to inhibitory substances, such as humic acids present in environmental samples.

This kit contains primers and a probe designed to detect a highly specific region of the mitochondrial DNA of Lissotriton vulgaris.


Detection as a service:

We offer the detection of Lissotriton vulgaris as a service. Visit the analysis page or contact us directly for more information about sampling, shipping, and pricing.


Kit contents:

• Primer/probe mix (10x)
  
• eTaq kit buffer
  
• eTaq DNA polymerase
  
• PCR-grade water
  
• Positive control

Sufficient for 200 reactions of 25 ul each.


Common species names:

• English: Smooth newt
  
• German: Teichmolch
  
• French: Triton ponctue
  
• Dutch: Kleine watersalamander
  

All kits are compatible with major qPCR platforms, including Bio-Rad, QuantStudio, and Applied Biosystems systems, and are suitable for FAM dye detection on most modern qPCR instruments. They are optimized for use on eDNA isolates purified using Sylphiums Environmental DNA isolation kit (#SYL002), but also work on isolates purified with other eDNA isolation methods/kits.

Our assays are validated for efficiency, inhibition resistance, and compliance with NPR 7394 quality standards. When combined with appropriate sampling methods, Sylphium eDNA detection kits provide a robust and dependable solution for species detection in environmental samples.


Why choose this kit?

• High resistance to inhibiting factors. Environmental DNA (eDNA) extracts often contain various substances that can inhibit qPCR. Standard qPCR mixes may be sensitive to these inhibitors.
  
• Strong fluorescence signal with low background noise. Environmental DNA extracts may contain naturally occurring autofluorescent compounds that can interfere with fluorescence measurements. A strong assay signal is therefore important to minimize the impact of background fluorescence.
  
• Highest possible sensitivity (1 DNA copy per reaction). Environmental water samples may contain only very small amounts of target DNA, making high analytical sensitivity essential.
  
• High specificity. Environmental DNA extracts contain billions of DNA fragments originating from bacteria, protozoa, plants, animals, and other organisms. During primer and probe design, not only closely related species must be considered; all available DNA sequences should be screened for potential nonspecific binding of the primers and probe.