The Microtus oeconomus (tundra vole) eDNA detection kit is able to detect the mammal species Microtus oeconomus in all droppings and soil samples worldwide and contains enough materials for 20 samples to be analysed in 8 fold (200 qPCR tests). The kit is based on the fast, sensitive, and proven primers/probe qPCR technique. The kit contains primers and a probe for detecting a highly specific sequence present on the mitochondrial DNA of the species Microtus oeconomus.
Be aware: Using soil as a sample has a chance of picking up ancient DNA of animals from years ago. DNA is highly stable connected to soil particles.
The tundra vole (Microtus oeconomus) or root vole is a medium-sized vole found in Northern and Central Europe, Asia, and northwestern North America, including Alaska and northwestern Canada. In the western part of the Netherlands, the tundra vole is a relict from the ice age and has developed to the subspecies Microtus oeconomus arenicola.
It has short ears and a short tail. Its fur is yellowish brown with paler sides and white underparts. They are about 18 cm (7.1 in) long with a 4 cm (1.6 in) tail and weigh about 50 grams (1.8 oz).
This species is found in damp tundra or moist meadows, usually near water. It makes runways through the surface growth in warm weather and tunnels through the snow in winter. It feeds on grasses, sedges and seeds.
Female voles have three to six litters of three to 9 young in a shallow burrow. The vole population in a given area can vary greatly from year to year.
It is active year-round. It also digs underground burrows where it stores seeds and roots, especially licorice root, for the winter. The species epithet oeconomus refers to this “economical” behaviour.
In English: Tundra vole
In German: Nordische Wühlmaus
In French: Campagnol nordique
In Dutch: Noordse woelmuis
The primers and probe are specially designed to be used with eDNA samples and have the following properties:
- Highest possible sensitivity (<1 DNA copies per reaction). Environmental water samples contain normally very low amounts of target DNA.
- Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto fluoresce substances that will interfere with the measurements. A strong fluorescence signal from the analyses is required for these kind of samples.
- 100% specificity. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc.
The kit is developed and optimized to be used on eDNA isolates purified using the eDNA isolation kit (#SYL002) from Sylphium molecular ecology.
Other eDNA isolation methods/kits can be used as well. Please contact us to get more information how to use the obtained isolates from other methods/kits to get reliable results with the Microtus oeconomus eDNA detection kit.
The kit contains materials for an 8 fold analyses on 20 samples including all necessary controls.
- Positive control (Microtus oeconomus DNA)
- 2x Sylphium qPCR mix (100 reactions) without primers and probes
- 2x Primer/probe mix (100 reactions) for detection of Microtus oeconomus (FAM dye)
- 1x Taq DNA polymerase (200 reactions)
- Protocol and primer/probe validation report