Ondatra zibethicus qPCR detection kit

496.00

Title Range Discount
regular price 0 - 4 0%
bulk discount 5 - 9 20%
bulk discount 10 - 19 30%
SKU: SYL119 Categories: , ,

Description

Ondatra zibethicus qPCR detection kitThe Ondatra zibethicus eDNA detection kit is able to detect the fish species Ondatra zibethicus in all water samples worldwide and contains enough materials for 200 reactions. The kit is based on the fast, sensitive, and proven Taqman® probe qPCR technique. The kit contains primers and a probe for detecting a highly specific sequence present on the mitochondrial DNA of the species Ondatra zibethicus. The Ondatra zibethicus qPCR detection kit is validated for for use in all waters worldwide.

Detection as a service
We offer the detection of Ondatra zibethicus also as a service. See our eDNA service website (in English or in Dutch) or contact us for more information about sampling, sending and pricing.

Species information

In English: Cottus perifretum/rhenanus
In German: Scheldegroppe/Rheingroppe
In French: Cottus perifretum/rhenanus
In Dutch: Muskusrat

Primer design
The primers and probe are specially designed to be used with eDNA samples and have the following properties:

  • Highest possible sensitivity (1 DNA copies per reaction). Environmental water samples contain normally very low amounts of target DNA.
  • Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto fluoresce substances that will interfere with the measurements. A strong fluorescence signal from the analyses is required for these kind of samples.
  • 100% specificity. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc.

The kit is developed and optimized to be used on eDNA isolates purified using the eDNA isolation kit (#SYL002) from Sylphium molecular ecology.
Other eDNA isolation methods/kits can be used as well. Please contact us to get more information how to use the obtained isolates from other methods/kits to get reliable results with the Ondatra zibethicus eDNA detection kit.

Kit contents

Ondatra zibethicus eDNA qPCR detection kit(SYL119)


The kit contains materials for 200 reactions.

  • Positive control (Ondatra zibethicus DNA)
  • 2x Sylphium qPCR mix (100 reactions) without primers and probes
  • 2x Primer/probe mix (100 reactions) for detection of Ondatra zibethicus (FAM dye)
  • 1x Taq DNA polymerase (200 reactions)
  • Protocol and primer/probe validation report

Downloads

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