Customize Consent Preferences

We use cookies to help you navigate efficiently and perform certain functions. You will find detailed information about all cookies under each consent category below.

The cookies that are categorized as "Necessary" are stored on your browser as they are essential for enabling the basic functionalities of the site. ... 

Always Active

Necessary cookies are required to enable the basic features of this site, such as providing secure log-in or adjusting your consent preferences. These cookies do not store any personally identifiable data.

No cookies to display.

Functional cookies help perform certain functionalities like sharing the content of the website on social media platforms, collecting feedback, and other third-party features.

No cookies to display.

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics such as the number of visitors, bounce rate, traffic source, etc.

No cookies to display.

Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors.

No cookies to display.

Advertisement cookies are used to provide visitors with customized advertisements based on the pages you visited previously and to analyze the effectiveness of the ad campaigns.

No cookies to display.

Environmental DNA isolation kit

83.001,149.00

  • eDNA isolation kit for water, feces and other environmental samples.
  • Optional: Internal positive control probe based qPCR quantification kit with all components of the eDNA master mix
Title Range Discount
regular price 0 - 4 0%
bulk discount 5 - 9 10%
bulk discount 10 - 19 20%
83.00
SKU: SYL002/20/000 Category:

eDNA Isolation Kit – SYL002

The eDNA Isolation Kit (SYL002) is designed for the rapid and efficient isolation of environmental DNA (eDNA) from water samples. Ideal for both qualitative and quantitative PCR amplification, this kit ensures a high yield of pure eDNA ready for use in qPCR. The entire isolation procedure takes approximately 2 hours, and the isolated DNA is dissolved in TE buffer, making it immediately ready for analysis.

This kit is perfectly suited for extracting and isolating eDNA from samples preserved with the eDNA Dual Filter Capsule (SYL009) and is compatible with all associated products. For a step-by-step guide on how to extract preserved eDNA from the capsule and proceed with the isolation, refer to the instruction protocol.

Optional: Internal Positive Control (IPC) Quantification Kit

All Sylphium sample sets include an internal positive control (IPC) in the preservation solution. This internal control consists of a small piece of synthetic xenobiotic DNA, which is unknown in the aquatic environment and will not interfere with the detection of target organisms. The IPC serves as an efficiency check for the DNA isolation procedure and a quality and purity check of the isolated DNA. The IPC quantification kit can be ordered separately and is quantified through the FAM channel.

Watch our tutorial video for a visual guide on using the eDNA Isolation Kit.

Kit Contents – DNA Isolation Kit

  • S1: Internal negative control (identical to Sample Preservation Buffer from Sylphium’s sampling sets)
  • S2: Phase Separation Solution
  • S3: Precipitation Buffer
  • S4: Precipitation Solution
  • S5: Wash Solution
  • S6: eDNA Conservation Buffer

Kit Contents – Internal Positive Control qPCR Detection Kit (Optional)

  • Positive Control
  • eTaq qPCR Mastermix
  • Primer/Probe Mix for Detection of the Internal Positive Control (FAM dye)
  • eTaq DNA Polymerase

Required Equipment and Consumables

  • qPCR Machine Capable of FAM Dye Detection (optional)
  • Microcentrifuge (13,000 x g)
  • Pipettors
  • -20 °C Freezer
  • PCR Cooling Rack (optional)
  • Centrifuge for 15 ml Tubes
  • Incubator or Heat Block at 55 °C
  • 15 ml Tubes with Cap
  • 2 ml Tubes with Lid
  • 96 Wells qPCR Plates + Seal Cover (optional)

Relevant Downloads

Shopping Cart