Complete eDNA sampling sets (20x)

€293.00

One box contains 20 complete sets.


Additional:

eDNA can be isolated clean, efficient, fast and affordable with the SYL002 – environmental DNA isolation kit. Optional with this kit is the IPC detection qPCR detection kit.


 

Title Range Discount
regular price 0 - 4 0%
bulk discount 5 - 9 5%
bulk discount 10 - 49 10%
bulk discount 50 - 99 15%
SKU: SYL009 Category:

Description

The eDNA sampling set includes the eDNA Dual Filter Capsule and valves designed for quick, easy, and clean sampling. The eDNA Dual Filter Capsule is specially crafted for on-site filtration of large volumes of environmental water, minimizing the risk of cross-contamination. Its enclosed filter membranes feature an optimal pore size (0.8 µm) for efficient environmental DNA (eDNA) filtration, preventing rapid clogging. The compact design incorporates two separate membranes, offering a substantial filtering surface area.

After use, the eDNA Dual Filter Capsule can be securely closed with ‘luer lock caps’ and is suitable for in-capsule lysing and preservation, with storage possible at room temperature. During eDNA extraction, all solid residues remain on the membrane, ensuring uninterrupted downstream processing. The eDNA sampling set complies with all requirements outlined in CEN/TC 230 – Water analysis – N 1229

DNA Dual Filter Capsule properties

  • Filter membrane: Poly ethylene sulfon (PES)
  • Possible pore sizes: 0.22 – 0.45 – 0.8 – 1.2 – 5 µm
  • Max inlet pressure: 1 Bar (45psi)
  • In and outlet fittings: Luer-lock female
  • Size: 108 mm x 52 mm x 130 m (L x W x H)
  • Cartridge material: Non-toxic Acrylonitrile butadiene styrene (ABS)
  • Sterilization method: Gamma irradiation

Set contents (20 sets per box)

  • eDNA Dual filter capsule with valve connector
  • 60 ml syringe
  • 3.5 ml Sample preservation solution* in 5 ml syringe. (with a xenobiotic internal positive control)
  • luer-lock male caps (2x)
  • Storage bag

* Extensive stability tests have demonstrated that the DNA concentration in the preservation solution remains stable for at least two years. These tests employed an internal positive standard to verify the integrity and consistency of the eDNA concentration. Furthermore, potential contaminants from the water matrix were monitored, showing no significant effects on the buffer’s efficacy after three months. This ensures that the buffer not only preserves the eDNA effectively but also maintains its purity and quality over prolonged storage periods.

(Optional) equipment and disposables

  • Sampling rod (SYL003)
  • Waste bag

Documents:

 

Folder with more details aboit the eDNA sampling set
Download the folder here!

eDNA sampling set:

  • Easy to use: Easy (sub)sampling without pooling
  • High yield: Large membrane surface and optimal pore size for eDNA filtration
  • No contamination: Enclosed filter membranes and in-capsule conservation
  • Clean eDNA isolates: In-capsule removal of PCR inhibitors during filtration and extraction

 

Large volumes and high eDNA yields:

In comparison to commonly used filtering methods in eDNA studies, the eDNA Dual Filter Capsule is able to filter nearly 10 times more water and eDNA than a fi lter funnel and even 20 times more than a Sterivex ™ filter. This results in 10 and 20 times more eDNA, respectively.
Maximum filtration and eDNA recovery from envirmental samples with the eDNA sampling set eith Dual Filter Capsule

 

 

 

 

 

 

 

 

 

 

 

 

Specs of the eDNA sampling set:

  • Filter unit type: Closed capsule
  • Effective Filter Area: 69 cm2 (dual filter system)

Additional information

Weight 80 g
Dimensions 7 × 5 × 5 cm
Membrane type

0.22 µM PES (microbial filter), 0.45 µM PES (eDNA filter), 0.8 µM PES (eDNA filter), 1.2 µM PES filter, 5 µM PES filter

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