SYL002 – Environmental DNA isolation kit

79.00779.00

  • eDNA isolation kit for water, feces and other environmental samples.
  • Optional: Internal positive control probe based qPCR detection kit.
  • Manual
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SKU: SYL002 Category:

Description

The Environmental DNA Isolation Kit is used to quickly and efficiently isolate environmental DNA from water samples. The Environmental DNA Isolation Kit procedure is suitable for qualitative and quantitative PCR amplification. The whole procedure for isolation of total eDNA takes about 2 hours. The isolated DNA will be dissolved in TE and is ready to use in qPCR.

The kit is perfectly suited for eDNA extraction and isolation from the eDNA Dual Filter membrane. See a short tutorial how to extract preserved eDNA from the capsule and how to proceed. The complete procedure is described in the instruction protocol.

Internal positive control qPCR detection kit (Optional):
The isolation kit contains an internal positive control (IPC). The internal control consists of a small piece of synthetic DNA, present in the conservation buffer. The chosen DNA sequence of the positive control is unknown to the aquatic environment and will not interfere in any detection of target organisms. The Internal positive control is an efficiency control of the DNA isolation procedure and a quality and purity control of the isolated DNA. The IPC can be detected with an probe based qPCR detection kit via the FAM channel. A positive signal should be obtained from this control in all cases (reactions). A negative signal indicates inhibiting substances in the eDNA isolate or a failure during isolation. This optional IPC detection kit (SYL113) can be ordered together with the isolation kit.

 

Kit contents DNA isolation kit

  • S1: Internal negative control
  • S2: Phase separation solution
  • S3: Precipitation buffer
  • S4: Precipitation solution
  • S5: Wash solution
  • S6: eDNA conservation buffer

 

Kit contents Internal positive control qPCR detection kit (Optional)

  • Positive control
  • eTaq qPCR mastermix
  • Primer/probe mix for detection of the internal positive control (FAM dye)
  • eTaq DNA polymerase

 

Equipment and consumables required

  • qPCR machine  capable for detection of the FAM dye (optional)
  • Microcentrifuge (13,000 x g)
  • Pipettors
  • -20 °Freezer
  • PCR cooling rack (optional)
  • Centrifuge for 15 ml tubes
  • Incubator or heat block at 55 °C
  • 15 ml tubes with cap
  • 2 ml tubes with lit
  • 96 wells qPCR plates + seal cover (optional)

 

Downloads

Additional information

Weight 80 g
Dimensions 7 × 5 × 5 cm
Kit size:

20 isolates, 50 isolates, 100 isolates

Including internal positive control

No, Yes

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