Sylphium Molecular Ecology offers a wide range of fully validated, species-specific eDNA qPCR assays for the detection of both aquatic and terrestrial organisms. These assays provide exceptionally high sensitivity, specificity, and reproducibility for identifying organismal DNA in water, feces, and soil samples.
All analyses performed by Sylphium are fully validated, published, and carried out according to strict quality assurance protocols, based on NPR 7394 (General principles for quality assurance in molecular biology research on microorganisms and environmental DNA). Results are delivered in a clear and detailed report that includes all validation checks and quality controls (Example analysis report). For information on pricing and turnaround times, click here.
The table below lists all currently available and fully validated species-specific eDNA qPCR assays. This list will continue to expand as new assays are validated.
| Species | English name | Status | Available as a kit |
| Alexandromys oeconomus | Tundra vole | Validated | SYL109 |
| Anatoxine toxin gene cluster | Anatoxine toxin gene cluster | Validated | SYL122 |
| Anguilla anguilla | European eel | Validated | SYL135 |
| Anisus vorticulus | Lesser ramshorn snail | Validated | SYL128 |
| Aeshna viridis | Green hawker | Validated | SYL130 |
| Cottus perifretum/rhenanus | Bullhead | Validated | SYL143 |
| Cylindrospermopsine gene cluster | Cylindrospermopsine gene cluster | Validated | SYL124 |
| Enterococcus | Enterococcus | Validated | SYL156 |
| Epidalea calamita | Natterjack toad | Validated | SYL106 |
| Escherichia coli | Escherichia coli | Validated | SYL134 |
| Hyla arborea | European tree frog | Validated | SYL150 |
| Ichthyosaura alpestris | Alpine newt | Validated | SYL166 |
| Lepomis gibbosus | Pumpkinseed | Validated | SYL101 |
| Lissotriton vulgaris | Smooth newt | Validated | SYL149 |
| Lota lota | Burbot | Validated | SYL115 |
| Microcystine toxin gene cluster | Microcystine toxin gene cluster | Validated | SYL102 |
| Misgurnus fossilis | European weatherfish | Validated | SYL159 |
| Natrix maura | Viperine snake | Validated | SYL127 |
| Neomys fodiens | Eurasian water shrew | Validated | SYL116 |
| Ondatra zibethicus | Muskrat | Validated | SYL119 |
| Pathogenic Leptospira | Pathogenic Leptospira | Validated | SYL131 |
| Pelobates fuscus | Common spadefoot | Validated | SYL103 |
| Rana arvalis | Moor frog | Validated | SYL104 |
| Rutilus rutilus | Common roach | Validated | SYL139 |
| Thaumetopoea processionea | Oak processionary | Validated | SYL110 |
| Trichobilharzia sp. | Swimmer’s itch | Validated | SYL108 |
| Triturus cristatus | Northern crested newt | Validated | SYL114 |
The list above includes only fully validated assays. If you are interested in a species not yet available, please contact us — additional assays can be developed upon request.
In addition to offering these analyses in our own service laboratory, many of these tests are also available as ready-to-use kits. These kits can be used in your own eDNA laboratory, and Sylphium Molecular Ecology provides training and support for setting up and running eDNA workflows.