Description
The Environmental DNA Isolation Kit is used to quickly and efficiently isolate environmental DNA from water samples. The Environmental DNA Isolation Kit procedure is suitable for qualitative and quantitative PCR amplification. The whole procedure for isolation of total eDNA takes about 2 hours. The isolated DNA will be dissolved in TE and is ready to use in qPCR.
The kit is perfectly suited for eDNA extraction and isolation from the eDNA Dual Filter membrane. See a short tutorial how to extract preserved eDNA from the capsule and how to proceed. The complete procedure is described in the instruction protocol.
Optional: internal positive control (IPC) quantification kit
All Sylphium sample sets contain an internal positive control (IPC) in the preservation solution. The internal control consists of a small piece of synthetic xenobiotic DNA. The positive control DNA sequence selected is unknown in the aquatic environment and will not interfere with the detection of target organisms. The internal positive control is an efficiency check of the DNA isolation procedure and a quality and purity check of the isolated DNA. Optionally, the Internal Positive Control (IPC) quantification kit can be ordered along with the eDNA Isolation Kit. The IPC is quantified through the FAM channel.
Kit contents DNA isolation kit
- S1: Internal negative control (identical to Sample preservation buffer from the sampling sets of Sylphium)
- S2: Phase separation solution
- S3: Precipitation buffer
- S4: Precipitation solution
- S5: Wash solution
- S6: eDNA conservation buffer
Kit contents Internal positive control qPCR detection kit (Optional)
- Positive control
- eTaq qPCR mastermix
- Primer/probe mix for detection of the internal positive control (FAM dye)
- eTaq DNA polymerase
Equipment and consumables required
- qPCR machine capable for detection of the FAM dye (optional)
- Microcentrifuge (13,000 x g)
- Pipettors
- -20 °Freezer
- PCR cooling rack (optional)
- Centrifuge for 15 ml tubes
- Incubator or heat block at 55 °C
- 15 ml tubes with cap
- 2 ml tubes with lit
- 96 wells qPCR plates + seal cover (optional)
Relevant downloads:
