The Rana arvalis (moor frog) eDNA detection kit is able to detect the amphibian species Rana arvalis in all waters worldwide and contains enough materials for 20 samples to be analysed in 8 fold (200 qPCR tests). The kit is based on the fast, sensitive, and proven Taqman® probe qPCR technique. The kit contains primers and a probe for detecting a highly specific sequence present on the mitochondrial DNA of the species Rana arvalis.
The moor frog (Rana arvalis) is a slim, reddish-brown, semiaquatic amphibian native to Europe and Asia. It is a member of the family Ranidae, or true frogs. The moor frog’s scientific name, Rana arvalis means “frog of the fields”. This is a small frog, characterized by an unspotted belly, a large, dark ear spot, and — often, not always — a pale stripe down the center of the back. They are generally described as a reddish-brown, but can also be yellow, gray, or light olive. Their bellies are white or yellow and they have a “bandit-like” black stripe going from their nose to their ears. They vary from 5.5 to 6.0 cm long, but can reach up to 7.0 cm in length, and their heads are more tapered than those of the Common frog (Rana temporaria). The skin on their flanks and thighs is smooth, and the posterior part of their tongues is forked and free. They have horizontal pupils, their feet are partially webbed, and their back legs are shorter than those of other species of frogs. The males are different from the females because of the nuptial pads on their first fingers and their paired guttural vocal sacs. (source: wikipedia)
In English: Moor frog
In German: Moorfrosch.
In French: grenouille des champs or grenouille oxyrhine
In Dutch: Heikikker.
The primers and probe are specially designed to be used with eDNA samples and have the following properties:
- Highest possible sensitivity (1 DNA copies per reaction). Environmental water samples contain normally very low amounts of target DNA.
- Strong fluorescence signal with low background noise. Isolated environmental samples contain residues of naturally occurring auto fluoresce substances that will interfere with the measurements. A strong fluorescence signal from the analyses is required for these kind of samples.
- 100% specificity. Isolated DNA from environmental samples contains billions of DNA fragments from bacteria, protozoa, plants, animals, etc.
The kit is developed and optimized to be used on eDNA isolates purified using the eDNA isolation kit (#SYL002) from Sylphium molecular ecology.
Other eDNA isolation methods/kits can be used as well. Please contact us to get more information how to use the obtained isolates from other methods/kits to get reliable results with the Rana arvalis eDNA detection kit.
The kit contains materials for an 8 fold analyses on 20 samples including all necessary controls.
- Positive control (Rana arvalis DNA)
- 2x Sylphium qPCR mix (100 reactions) without primers and probes
- 2x Primer/probe mix (100 reactions) for detection of Rana arvalis (FAM dye)
- 1x Taq DNA polymerase (200 reactions)
- Protocol and primer/probe validation report