The SYL152 Bombina variegata Detection Kit is designed for precise, trustworthy environmental DNA (eDNA) analysis.
Powered by our advanced eDNA qPCR Hot Start Mix, this kit delivers exceptional sensitivity, robustness, and accuracy in qPCR detection assays.
It performs consistently even in challenging environmental samples containing inhibitors such as humic acids.
Why Choose This Kit?
- 100% specificity – Targets Bombina variegata with minimal cross-reactivity.
- Ultra-high sensitivity – Capable of detecting a single DNA copy per reaction.
- Inhibitor resistant – Reliable performance in real-world environmental conditions.
- Strong fluorescence signal – Low background noise for clear and accurate detection results.
- Validated performance – Achieves ≥ 95% detection probability when paired with correct sampling protocols.
Detection as a Service
No access to qPCR equipment? We offer professional eDNA detection services for Bombina variegata! Visit our eDNA service website (available in English and Dutch) or contact us for information on sampling, shipping, and pricing.
Common Names
- English: Yellow-bellied toad
- German: Gelbbauchunke
- French: Sonneur à ventre jaune
- Dutch: Geelbuikvuurpad
Kit Compatibility & Storage
- Validated on major qPCR platforms, including Bio-Rad, QuantStudio, and Applied Biosystems.
- Compatible with FAM dye detection – Works with most modern qPCR machines.
- Optimized for use with the Sylphium Molecular Ecology eDNA Isolation Kit (#SYL002), but suitable for other eDNA isolation methods.
- Storage conditions:
- -80°C for long-term stability (>6 months).
- -20°C for short-term use.
- Guaranteed stable for 1 year after delivery when stored according to recommendations.
Kit Contents
- Primer/probe mix (10x) for Bombina variegata detection (FAM dye).
- eDNA qPCR Master Mix, containing:
- eTaq DNA polymerase
- eTaq qPCR mix
- PCR-grade water
- Positive control for quality assurance.
- Sufficient for 200 reactions (25 µl each).
Relevant Documents
Questions? Contact us at info@sylphium.com
