The SYL153 Alytes obstetricans Detection Kit is engineered for accurate and consistent environmental DNA (eDNA) detection.
Using our advanced eDNA qPCR Hot Start Mix, this kit delivers exceptional sensitivity, robustness, and accuracy in qPCR-based detection assays.
It performs reliably even in challenging environmental samples containing inhibitory substances such as humic acids.
Why Choose This Kit?
- 100% specificity – Targets Alytes obstetricans with minimal cross-reactivity.
- Ultra-high sensitivity – Detects a single DNA copy per reaction.
- Inhibitor resistant – Ensures dependable results in complex environmental matrices.
- Strong fluorescence signal – Low background noise for clear and accurate detection.
- Validated performance – Achieves ≥ 95% detection probability under recommended sampling conditions.
Detection as a Service
No qPCR equipment available? We provide professional eDNA detection services for Alytes obstetricans! Visit our eDNA service website (available in English and Dutch) or contact us for sampling, shipping, and pricing information.
Common Names
- English: Common midwife toad
- German: Geburtshelferkröte
- French: Alyte accoucheur
- Dutch: Vroedmeesterpad
Kit Compatibility & Storage
- Validated on major qPCR platforms, including Bio-Rad, QuantStudio, and Applied Biosystems.
- Compatible with FAM dye detection – Suitable for most modern qPCR instruments.
- Optimized for use with the Sylphium Molecular Ecology eDNA Isolation Kit (#SYL002), but compatible with other eDNA extraction methods.
- Storage conditions:
- -80°C for long-term stability (>6 months).
- -20°C for short-term use.
- Guaranteed stable for 1 year after delivery when stored properly.
Kit Contents
- Primer/probe mix (10x) for Alytes obstetricans detection (FAM dye).
- eDNA qPCR Master Mix, containing:
- eTaq DNA polymerase
- eTaq qPCR mix
- PCR-grade water
- Positive control for quality assurance.
- Sufficient for 200 reactions (25 µl each).
Relevant Documents
Questions? Contact us at info@sylphium.com
